Experimental studies on hematopoiesis reconstitution by using of transplantation of gene-modified bone marrow cells / 中华器官移植杂志
Chinese Journal of Organ Transplantation
;
(12): 212-214, 1999.
Article
in Chinese
| WPRIM
| ID: wpr-387517
ABSTRACT
Objective To explore the biological features of hematopoiesis reconstitution by using genetic marking.Methods NeoR gene was transduced into bone marrow(BM)cells of mouse mediated by liposome.Then these cells were transplanted into lethally irradiated recipients.The hematopoiesis reconstitution was observed and the marker gene in spleen and BM cells of recipients after hematopoiesis reconstitution was examined.Results The transplanted recipients remained alive and healthy.But the irradiated mice with no transplantation died from BM aplasia soon.Meanwhile,the cells from spleen and BM of transplanted mice could be alive in G418 system,and contained the DNA fragments of NeoR gene by PCR.Conclusion Gene-modified BM cells could be used to reconstitute hematopoiesis successfully and express the foreign gene to some extent stably.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Organ Transplantation
Year:
1999
Type:
Article
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