Effects of isoflurane on expression of receptor for advanced glycation endproducts in hippocampus in rats / 中华麻醉学杂志

ABSTRACT

Objective To investigate the effects of isoflurane on receptor for advanced glycation endproducts (R(A)GE) expression in the hippocampus in rats. Methods Forty-five male 4-month-old and 45 male 24-month-old rats were used in this study. The animals were divided into 2 age groups ( n = 45 each) the aged group (group O) and the adult group (group A). Each group was further divided into 3 subgroups ( n = 15 each)Ⅰ control subgroup (group OC,AC) inhaled 30% O2 in air; 1 single isoflurane inhalation subgroup (group OS,AS) inhaled 1.5 % isoflurane for 2 h and Ⅲ repeated isoflurane inhalation subgroup (group OR, AR) inhaled 1.5 % isoflurane 2 h per day for 3 days. One day after isoflurane inhalation, learning and memory function was assessed using Morris water maze test in 8 animals in each subgroup. The rest of each subgroup were killed and their hippocampi were immediately isolataed for detection of RAGE mRNA and protein expression by RT-PCR and immuno-histochemistry. Results The cognitive function was impaired after signle or repeataed isoflurane anesthesia as compared with control animals in both aged and adult groups. The expression of RACE mRNA and protein in hippocampus was significantly increased after either single or repeated isoflurane anesthesia in aged group but only after repeated isoflurane anesthesia in adult gpoup. There was no significant difference in RAGE mRNA and protein expression in the hippocampus between control and single isoflurane inhalation animals in adult group. Conclusion Isoflurane can reduce learning and memory function in both aged and adult rats by increasing RAGE expression in hippocampus especially in aged rats.