Patent-blue-liposomes for lymphography and the detection of their biological property / 国际外科学杂志

ABSTRACT

Objective To prepare patent-blue-lipesomes(PB-LPS),a lymph-mapping developer.Methods PB was encapsulated in the liposomes using the passive loading method.The female Wistar rats were injected subcutaneously with the PB-LPS,PB,blank liposomes or isotonic rmtrium chloride at the heel solos,to study the stained situation of the lymph nodes and chemical analysis oftheir ALT,AST,BUN,Cr.Results The lymph nodes were obviously blue staining after injected PB-LPS or PB,the blue smimng ratios were 40%,20%(P<0.05),and the effect of PB-LPS on tracing of lymphy nodes was better than PB.No significant difference was found in ALT,AST,BUN and Cr.Conclusion The PB-LPS can be successfully prefabricated with such advantage as avirulence and high entrapment efficiency by passive loading method.