The expression of hypermethylated secreted frizzled-related protein 2 gene in eolorectal cancer / 中华普通外科杂志

ABSTRACT

Objective To analyze the relation of hypermethylated secreted frizzled-related protein 2 (SFRP2) gene in feces and peripheral blood of colorectal cancer(CRC) patient with Dukes stages and histological types,and to evaluate SFRP2 gene for screening and judging the pathogenetic condition of CRC.Methmls Methylation-specific PCR assay was performed to analyze SFRP2 gene promoter methylation status in a blinded fashion in tumor tissues,stool and pefipheral blood samples taken from 169 CRC patients,63 patients with adenoma,46 with hyperplastic polyp and 30 endoscopically normal subjects.Results SFRP2 gene Was hypermethylated in 88.2%.65.1%and 45.7% of CRC.adenoma and non-adenomatous polyp tissues,in 84.0%,46.0% and 32.6%of corresponding fecal samples,and in 66.9%,6.4%and 2.2% of corresponding peripheral blood samples,respectively.In contrast,no methylated SFRP2 gene was detected in mucosal tissues and peripheral blood of normal controls,while two cases of matched fecal samples from normal controls were detected with hypermethylated SFRP2.Moreover,no significant correlation were observed between SFRP2 hypermethylation and clinicopathological features including sex,age,size,location and Dukes stage,but there was a significant correlation between SFRP2 hypermethylation and tumor difierentiation and infiltration.SFRP2 hypermethylation in peripheral blood of CRC patients was positively correlated with the Dukes stage. Condusions Hypermethylation of SFRP2 gene in feces works as a molecular biomarker for CRC screening.The hypermethylation of SFRP2 gene is positively related with Dukes stage.