Red fluorescent transplantation tumor model of mouse bladder carcinoma and fluoroscopic image study / 中华泌尿外科杂志

ABSTRACT

Objective To explore molecular fluorescence imaging features of the growth and metastasis of DsRed-marked mouse bladder carcinoma. Methods The study used lipofectamine 2000 transfection method,transferred on the BTT739 cells with plasmid chickenβ-actin-DsRed-Neo vector.The stably expressing BTT739-DsRed monoelonal cells were got with G418 selection.It randomly divided the 615 mouse of 24 into three groups,injected cell suspension on the hindlimb,the first and second group with BTT739-DsRed cell and the third group with BTT739 cell to found xenograft roodel.MAESTRO imager recorded fluorescence images of the growth and metastasis of the tumors in vivo and the fluorescence intensity was measured.The excitation wavelength was 560-580 nm,emission wavelength was 590-610 nm and exposure time was 5000 ms.After continuous observation of 4 weeks,every week killed the mouse of the second group and cut into image,made records of the red fluorescent mouse bladder cancer xenograft model,measured the tumor size and fluorescence sighal values; analyzed the relations between the tumor size and fluorescence signal values as well as between the whole image and cut image. Results DsRed tumor could be observed at the first week. Central local fluorescence loss could be detected at the second week, pathologically confirmed necrotic tumor tissue and a little connective tissue. At the fourth week, a local lymph node metastasis could be observed with no distant metastasis. The measured values of fluorescent signal were as follows (89±18), (122±55), (133±69), (715±343)counts; the tumor size were as follows (13±4), (45±22), (83±29), (253±67)mm2. The whole body image of tumor size were as follows (12± 3),(50±23), (90±29), (290±74)mm2. The cut image of tumor size were as follows (12±5), (72±30), (141±43), (524±237)mm2. The tumor size and fluorescent signal values reflect positive linear correlation with 0. 74 coefficient (t= 3. 97, P<0.05), whole body imaging and cut image reflect positive linear correlation with 0. 97 coefficient (t=10, P<0.05). The whole body image of tumor size was (70. 85±17.13) % of cut image. Conclusions Red fluorescent mouse bladder cancer xenograft model could observe the growth and metastasis of the tumor intuitively, continuously, and sensitively.As the tumor increased, the fluorescence range also increased, the fluorescence disappeared after tumor necrosis, the expression of the red fluorescent transferred after the metastasis of the tumor.