Association of polymorphism of peptidylarginine deaminase-4 gene and rheumatoid arthritis / 中华风湿病学杂志

ABSTRACT

Objective To investigate the distribution characteristics of single nucleotide polymorphisms (SNPs) of peptide arginine deaminase4 (PADI4)-94 and PADI4-104 with rheumatoid arthritis(RA)and study the association between PADI4-94/PADI4-104 SNPs and RA susceptibility.Methods PCR-LDR was used to identify the gene and genotype of 116 rheumatoid arthritis patients and 100 healthy controls and calculated the frequencies.Chi-square test was used to analyze the statistical significance.The presence of anti-PADI4 and PADI4 protein in the peripheral blood of patients with RA was examined using enzyme-linked three kinds of genotypes (A/A,G/G and A,G),PADI4-94 allele A,G and A/A,C/G and A/G genotype frequencies in RA group and healthy controls were 0.460/0.392,0.540/0.608,0.204/0.186,0.283/0.402 and 0.513/0.412,respectively.There was no significant difference between the two groups (X2=1.996,P=0.157;X2=3.407,P=0.182);PADI4-104 allele A,G and A/A,G/G and A/G genotype frequencies in RA group and healthy controls were 0.412/0.345,0.588/0.655,0.150/0.144,0.327/0.454 and 0.522/0.402,respectively.There no significant differences between PADI4-94/PADI4-104 genotypes and ESR,DAS28 score,CRP,anti-CCP antibodies,PADI4 protein,and anti-PADI4 antibodies(P values were 0.46/0.67,0.62/0.57,0.12/0.23,0.81/0.43,0.78/0.75,0.38/0.31).Conclusion The SNPs of PADI4-94 and PADI4-104 in Chinese RA population can be identified,but they may not be the susceptibility genotypes of RA.