Newly Identified TLR9 Stimulant, M6-395 Is a Potent Polyclonal Activator for Murine B Cells
Immune Network
;
: 27-32, 2012.
Article
in English
| WPRIM
| ID: wpr-39027
ABSTRACT
BACKGROUND:
Toll-like receptors (TLRs) have been extensively studied in recent years. However, functions of these molecules in murine B cell biology are largely unknown. A TLR4 stimulant, LPS is well known as a powerful polyclonal activator for murine B cells.METHODS:
In this study, we explored the effect of a murine TLR9 stimulant, M6-395 (a synthetic CpG ODNs) on B cell proliferation and Ig production.RESULTS:
First, M6-395 was much more potent than LPS in augmenting B cell proliferation. As for Ig expression, M6-395 facilitated the expression of both TGF-beta1-induced germ line transcript alpha (GLTalpha) and IL-4-induced GLTgamma1 as levels as those by LPS and Pam3CSK4 (TLR1/2 agonist) a certain Ig GLT expression is regarded as an indicative of the corresponding isotype switching recombination. However, IgA and IgG1 secretion patterns were quite different--these Ig isotype secretions by M6-395 were much less than those by LPS and Pam3CSK4. Moreover, the increase of IgA and IgG1 production by LPS and Pam3CSK4 was virtually abrogated by M6-395. The same was true for the secretion of IgG3. We found that this unexpected phenomena provoked by M6-395 is attributed, at least in part, to its excessive mitogenic nature.CONCLUSION:
Taken together, these results suggest that M6-395 can act as a murine polyclonal activator but its strong mitogenic activity is unfavorable to Ig isotype switching.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligodeoxyribonucleotides
/
Recombination, Genetic
/
Immunoglobulin A
/
Immunoglobulin G
/
B-Lymphocytes
/
Immunoglobulin Class Switching
/
Cell Proliferation
/
Toll-Like Receptors
/
Germ Cells
Language:
English
Journal:
Immune Network
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS