Observation of metastasis of colon adenocarcinoma Iovo cells in vitro under laser scanning confocal microscope / 国际生物医学工程杂志

ABSTRACT

Objective To explore the feasibility of applying laser scanning confocal microscopy to investigate the metastatic characteristic of tumor cells in vitro. Methods The confocal microscope combined with transwell assay was employed to observe the invasion and migration of the metastatic colon cancer ]ovo cells when stimulated by IL-8. Results The results show that the number of invasive lovo cells in the stimulated group was much higher than the unstimulated group (P<0.05). The migration way of lovo cells observed under the confocal microscopy was ameba like movement. These findings suggest that the metastatic ability of lovo cells stimulated by IL-8 was enhanced. Conclusion The metastatic characteristic of colon adenocarcinoma lovo cells is similar to the chemotactic movement of leukocytes.