Activin A Stimulates Mouse APCs to Express BAFF via ALK4-Smad3 Pathway
Immune Network
;
: 196-202, 2011.
Article
in English
| WPRIM
| ID: wpr-39109
ABSTRACT
BACKGROUND:
B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and dendritic cells, and stimulates B cell proliferation, differentiation, survival, and Ig production. In the present study, we explored the effect of activin A on BAFF expression by APCs.METHODS:
To investigate the effect of activin A on BAFF expression by mouse APCs, we measured the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA.RESULTS:
Activin A markedly enhanced BAFF expression in mouse macrophages and dendritic cells at both the transcriptional and protein levels. SB431542, an activin receptor-like kinase 4 (ALK4) inhibitor, completely abrogated activin A-induced BAFF transcription. Furthermore, overexpression of DN-Smad3 abolished activin-induced BAFF expression at the transcriptional and protein levels.CONCLUSION:
These results demonstrate that activin A can enhance BAFF expression through ALK4-Smad3 pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Benzamides
/
Dendritic Cells
/
Activins
/
Activin Receptors
/
Cell Proliferation
/
Dioxoles
/
Macrophages
Limits:
Animals
/
Humans
Language:
English
Journal:
Immune Network
Year:
2011
Type:
Article
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