Study of prevalence of mixed species malaria infection and distinguishment of recrudescence from re-infection in Gia Lai province by the use of nested polymerase chain reaction (PCR)

ABSTRACT

Application of PCR technique to survey component and mechanism of 4 human malaria parasites in Gia Lai province. These used bait couples were specificity for P.falciparum, P.vivax, P.malariae and P.ovale. The results showed that there were the existance of 4 human malaria parasite species, of which P.falciparum was 57.6%, combination rate of 2,3,4 parasites species was 54.76%. To distinguish the recurrent, re-infection based on the difference of alleles in locus gen. 3 genes for highly polymorphic antigens merozoite surface protein 1 (MSP1), merozoite surface protein 2 (MSP2), glutamate rich protein (GLURP). 11 patients were treated by fansidar had been recurrented after 14 days