Expression of phosphatidylinositol 3-kinase/protein kinase B signaling pathway in cervical cancer and clinical significance / 中国医师进修杂志

ABSTRACT

Objective To determine the expression of phosphatidyhnositol 3-kinase (PI3K), phosphorylated protein kinase B (P-AKT) and protein kinase B (AKT) in cervical cancer and explore the correlations with the genesis and development of cervical cancer. Methods Western blot was used to detect the expression of PI3K, P-AKT and AKT proteins in 66 cases of cervical tissues, including 33 cervical cancers, 23 cervical intraepithelial cancer, 10 normal cervical tissues, and analyzed the clinical significances according to clinical informations. Results In cervical cancers, cervical intraepithelial cancer and normal cervical tissues, the expression of PI3K protein were 1.3880±0.0435, 0.5330±0.0939, 0.2427±0.0888 and had significant differences among them, but there was no significant difference in the ratio of P-AKT and AKT, which were 5.8702±0.0543, 5.0755±0.0888, 3.8353±0.0056. The ratios of P-AKT and AKT had association with histological type, the ratios in squamous cancer of the cervix and in adenocancer and denosquamous cancer were 6.7823±0.7745 and 0.7621±0.0709, and the ratios were much higher in squamous cancer of the cervix than adenocancer and denosquamous cancer. Conclusions Overexpression of PI3K protein is involved in the occurrence of human cervical cancer. The ratio of P-AKT and AKT plays a more important role in squamous cancer of the cervix.