The level of GITR mRNA expression on peripheral blood monocytes of patients with primary biliary cirrhosis / 中华风湿病学杂志

ABSTRACT

Objective To detect the expression of GITR mRNA in peripheral blood mononuclear cells (PBMCs) of patients with primary biliary cirrhosis (PBC) and its relationship with the disease activity of PBC.Methods Real time quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) was used to examine GITR mRNA expression on peripheral blood monocytes of 44 active PBC, 34 stable PBC, 30.hepa-toma cases and 30 healthy controls. Results The mean GITR mRNA expression of active PBC (△Ct=10.5±