Expression of ED1 positive cell in glomerulosclerosis in rats / 中国医师杂志

ABSTRACT

Objective To assay the expression chinse of ED1 positive cell in focal segmental glomemlosclerosis in rats,we investigated the relationship between the infiltration of mononuclear phagocytes and the progression of glomendar sclerosis.Methods We used 12 Wistar ratswhichwere dividedintotwo groups.1est group and coutrol group.Themodel offocal segmental glomerursclerosiswas ulade by in jecting PAN 9 mg/100g body weights.The rats ofcontrol group were injected 3ml 0.9％80diuln chloride.The proteinuria,serum creatinine,fipi&and protein of the rats were examined.The rata were killed at the 20th week.All the kidneys were kept and nlade into pathologic slEun-pie.1mmunohistochemical method was applied to detect the protein expression of FIN and the EDI positive cell in renal tissue of all the rats,and the number of Edl positive cell W88 counted.The results were analyzed by SPSS.Results The proteinuria of the mts in FSGS model group was significantly increased,the serum lipid ofthem was also increased.The pathology changes of the rat renal in model group showed that a part of giomemli appeared focal segmental sclerosis or all glomerular sclerosis,and the extracelhlar matrix accumulated.In the renal of model rats,the amount of EDI positive cells was significantly higher than that in normal rats(P<O.05).The expression of FN in the rehal of model rats was significantly higher than that in the normal rats(P<0.05).111e mount ofEDl positive cells WaS significantly positively correlated WitII the expression of FN(P=0.002).The amount of Edl positive cells W88 significantly positively correlated with the pmteinuria of the rats(P=0.014).Conclusion Theinfiltration ofEDI positivecell contributed to the progression of glomerular sclerosis.