Chemosensitivity of pancreatic cancer cell in multicellular spheroids / 中华胰腺病杂志

ABSTRACT

Objective To investigate the feasibility of pancreatic cancer muhieellular spheroids in chemosensitivity study. Methods Pancreatic adenocarcinoma cell lines SW1990, PCT-3 and ASPC-1 were cultured in collagen gel. When the muhicellular spheroids were formed, the drug chemosensitivities of Fluorouracil ( 5-FU ), Gemzar ( GEM ) and Oxaliplatin ( OXA ) were detected by CD-DST and CCK-8 methods. The results were compared with those of scattered cells model. Results The chemosensitivities of three pancreatic lines in form of multicellular spheroids were significantly lower than those of scattered cells model (P < 0. 05). The inhibitory rates of 50 μg/ml 5-FU on SW1990, PCT-3, ASPC-1 were (53.96 ±4.32)% ,(58.49±5.98)%, (49.57±4.36)% ;the inhibitory rates of 25 μg/ml GEM on SWI990,PCT-3, ASPC-1 were (53.02 ± 4.06) %, (61.90 ± 4.80) %, (38.09 ± 4.88 ) % ;the inhibitory rates of 10 μg/ml OXA on SW1990, PCT-3, ASPC-1 were (57.33 ± 6.27 ) %, (50.90 ± 4.80) %, (47.26 ± 4.29) % ;which were significantly lower than those of scattered cells model ( P < 0.05 ). Conclusions In collagen gel, formation of the multicellular spheroids of pancreatic cells caused less chemosensitivity and promoted anticancer drug resistance, which was consistent with in vivo state.