A study on the HSV-2 Infection and Ki——67 expression of the cervical carcinoma and cervical intraepithelial ne oplasia / 中国基层医药

ABSTRACT

Objective To explore the herpes virus Ⅱ(HSV-2) infection and Ki-67 expression for the cervical carcinoma and cervical intraepithelial neoplasia(CN).Methods The polymerase chain reaction(PCR) and immunohistochemistry were applied to detect the HSV-2 and Ki-67 expressions in cancerous tissues of uterine cervix in 84 cases (67 scaly cell carcinoma,3 scaly adenocarcinoma,and 14 adenocarcinoma cases),CN in 35 cases,and normal cervical tissues in 13 cases.Results (1)The positive rote of HSV-2 is 40.2% (53/132),and the rate in cervical cancerous tissues,CN and normal cervical tissues were 48.8% (41/48) ,28.5% (10/35) and 15.4% (2/13) respectively.HSV-2 DNA expressions in cancerous tissue and CN were much higher than that of normal cervical tissues (P<0.01).(2)The Ki-67 positive cells were progressive increasing in the normal cervical tissues,CN and cervical cancerous tissues,thus showing that there were relations between the Ki-67 expression degree and tissue types.The HSV-2 and Ki-67 expressions were remarkably connected(P < 0.01).Conclusion There are HSV-2 infection ,close related between HSV-2 infection and Ki-67 expression in the tissues of cervical carcinoma and CN.And the cooperation of them may play an important role in the development of cervical carcinoma.