Delayed activation-induced T lymphocytes death in aplastic anemia: related with abnormal Fas system
The Korean Journal of Internal Medicine
;
: 41-46, 1998.
Article
in English
| WPRIM
| ID: wpr-39711
ABSTRACT
OBJECTIVES:
To quantitate apoptosis and Fas antigen expression of T lymphocytes by activation in aplastic anemia (AA) and compare with that of normal controls and completely-recovered AA, and to investigate the apoptotic sensitivity to anti-fas antibody of activated T lymphocytes in AA.METHODS:
We studied the expression of Fas antigen on fresh T lymphocytes of twenty patients with AA [13 newly diagnosed, 7 recorvered AA after immunosuppressive therapy (IST)], and investigated the activation-induced cell death (AICD) and Fas expression by activation [interleukin-2 (200 U/ml) and phytohemagglutinin (50 micrograms/ml)] in 5 newly-diagnosed AA, 5 normal controls and 5 AA in complete response (CR). Apoptotic sensitivity to anti-Fas antibody was assessed by the time-course kinetics of induction of cell death by anti-Fas antibody (500 ng/ml).RESULTS:
There was no significant difference of Fas antigen expression on freshly-isolated T lymphocytes among newly-diagnosed severe AA, normal control s and patients with AA in CR after IST. In normal controls, T lymphocytes death was greatly increased at 3 days of activation, and Fas antigen expression on T lymphocytes was increased above baseline at day 1 of activation. In contrast, in newly-diagnosed AA, T lymphocytes showed delayed cell death, which correlated with a slowed increase of Fas antigen expression by activation. Also, anti-Fa s antibody sensitivity of activated T lymphocytes was decreased in newly-diagnosed AA. In completely recovered AA, these abnormal AICD and Fas antigen expressions by activation were recovered to normal range.CONCLUSIONS:
Abnormal AICD plays a role in the immune pathophysiology of AA, and defective Fas system is involved in this process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
In Vitro Techniques
/
Lymphocyte Activation
/
T-Lymphocytes
/
Case-Control Studies
/
Apoptosis
/
Fas Receptor
/
Anemia, Aplastic
Type of study:
Observational study
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Internal Medicine
Year:
1998
Type:
Article
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