Effect of MMDB on the expression of TLR4 in cholestatic jaundice in rats / 国际外科学杂志

ABSTRACT

Objective To research the mechanism of impairment that cholestatic jaundice is treated as- sistly by MMDB. Methods Obstructive jaundice models with SD rats, which were divided into three time points randomly, namely 7,14,21 days,were established. In each time point, there were three groups(each six rats) sham operation(SO + NS) ; BDL + MMDB; (BDL + NS). The rats were sacrificed at different time point, in order that liver tissue and inferior vena cava blood were taken out. The concentration of serum glutamate transaminase(AST), albumin(ALB), and total bilirubin (TB) were measured with biochemistry; the expression of TLR4mRNA was measured by reverse transcription PCR. Results On the 7th day after BDL, serum TB,AST level in BDL + MMDB and BDL + NS group were upgrade compared with SO group, in which BDL + NS group were more obviously upgrade than BDL + MMDB group(P<0.01). There was differ- ent to compare between BDL + MMDB group and SO group(P <0.01). On the 14th, 21st day after BDL, serum TB, AST coNTents were to last upgrade in BDL + MMDB and BDL + NS group. It was opposite to the SO group(P <0. 01). Conclusion The level of TLR4 could be weaken by MMDB. It may relieve the im- pairment of liver in cholestatic jaundice by LPS.