Effect of recombinant human tumor necrosis factor receptor Ⅱ-Fc fusion protein on nuclear factor-κB protein expression and brain edema tranmalic brain injury in rats / 中国综合临床

ABSTRACT

Objective To investigate the effects of recombinant human tumor necrosis factor receptorⅡ-Fc fusion protein(rhTNFRFc ) on nuclear factor-κB(NF-κB)and tumor necrosis factor-α(TNF-α)protein expression in the rat brain tissue,and traumatic brain edema following acute traumatic brain injury(TBl).Methods The inju-ry rats were subjected to right lateral cortical impact injury caused by a free-falling object.In the rhTNFRFc group,rhTNFRFc was administered intraperitoneally(3.0 mg/kg)after 30 min of injury-and the rats of control and injury group administered with normal saline solution.The levels of TNF-α protein in rat brain were mensured by radio-im-munonssay(RIA).The NF-κB protein expression of rat brain was studied immunohistochemically.In the meantime,the water content of rat brain Was measured,and for the microscope and ultrastructure examinations by using electron-ic microscope respectively.Results Compared with control group,the levels of NF-κB and TNF-α protein and the water content of brain tissue were obvicously increased from 6 h following TBI(P<0.05,or P<0.01).Compared with injury group,the expression of NF-KB and TNF-α protein,and the brain water content were lower in rhTNFRFc group(P<0.05-P<0.01).The rhTNFRFc Can reduce the brain injury in electronic microscope examinations.Conclusion The expression of NF-κB and TNF-α,and the water content of rat brain increase significantly follow-ing acute traumatic brain injury.The rhTNFRFc can dramatically inhibit the expression of NF-κB and TNF-α,and alleviate rat brain edema after TBI.