Relationship between CX3CR1 polymorphisms and carotid artery stenosis / 中华神经科杂志

ABSTRACT

Objective To study the association of V249I and T280M polymorphisms of fractalkine receptor CX3CR1 with carotid artery stenosis (CAS). Methods 318 patients with CAS diagnosed using color Doppler ultrasound criteria were studied and compared with 292 subjects without CAS. V249I and T280M polymorphic genotypes of CX3CR1 were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and sequencing analysis. Results The genotypes of MM and TM were associated with reduced risk of CAS,the frequency of genotype MM + TM in the two groups being 22. 6% and 31.2% respectively ( OR = 0. 646, 95% CI 0. 451-0. 928, P = 0. 017), the frequency of M allele was significantly lower in patients with CAS than in those without CAS ( 13.8% and 19. 2% respectively, OR = 0. 677, 95%CI 0.499--0. 918,P=0. 010). No differences were observed in the Ⅱ, Ⅵ, or VV genotype, the frequency of genotype Ⅱ + Ⅵ in the two groups being 39. 0% and 43.8% respectively ( OR = 1. 012, 95% CI 0. 731-1. 403 ,P = 0. 940), the frequency of Ⅰ allele was significantly lower in patients with CAS than in those without CAS (28. 6% and 32. 7% respectively, OR = 0. 842, 95% CI 0. 660-1. 076, P = 0. 034). The genotypes of Ⅱ and Ⅵ in patients with stable plaques were more frequent than in vulnerable plaques( OR = 0. 610, 95% CI 0. 387-0. 962, P = 0. 033 ). Multiple Logistic regression analysis revealed that the genotypes of MM and TM were an independent risk factor for CAS ( OR = 1. 847,95% CI 1. 091- 3. 127 ,P = 0. 022). Conclusion The genotypes of MM and TM are independent risk factors for carotid artery stenosis, and the genotypes of Ⅱ and Ⅵ are associated with the stability of carotid artery plaques.