Expression changes of Cx37, Cx40 and Cx43 of superior mesenteric arteries and their significance following hemorrhagic shock in rats / 中华创伤杂志

ABSTRACT

Objective To observe the expression changes of Cx 37, Cx 40 and Cx 43 of superior mesenteric arteries (SMA) and their significance following hemorrhagic shock in rats. Methods SMA was removed from normal or shocked SD rats at different time points (immediately, 30 minutes, 1,2, 3 and 4 hours after shock) for detection of mRNA and protein expressions of Cx 37, Cx 40 and Cx 43. Fur- thermore, the effect of Cx 40 or Cx 43 (Cx 40 AODN or Cx 43 AODN) on SMA's vasomotor reactivity induced by myricetin or acetylcholine was studied by antisense oligndeoxynucleotide. Results The ex- pressions of Cx 37 and Cx 40 of SMA were gradually decreased within 2 hours after shock and significantly increased at 3 and 4 hours after shock. While expression of Cx 43 was gradually increased at 0. 5, 1 and 2 hours after shock and significantly decreased at 3 and 4 hours after shock. Cx 40 AODN significantly in- creased the vasomotor reactivity induced by myricetin or acetylcholine while Cx 43 AODN exerted an inh- ibitive effect. Conclusions Cot 40 and Cx 43 participate in regulating endothelium-dependent vasomo- tar reactivity following hemorrhagic shock. Cx 40 has an inhibitive effects on endothelium-dependent vaso- motor reactivity but Cx 43 can promote such reactivity.