Effects of aspirin on PCNA and cyclin D1 expressions in diethylnitrosamine induced hepatic cancer of the rat / 中华普通外科杂志

ABSTRACT

Objective To observe the effects of aspirin on PCNA and cyclin D1 expressions in diethylnitrosamine (DEN) induced hepatic cancer of the rat. Methods Sprague-Dawley (SD) rats were randomly divided into DEN group and DEN plus aspirin group. Each group was further divided into 8,12,16 week subgroups. The expressions of PCNA and cyclin D1 in hepatic tissue were examined with inmunohistochemistry. Results In DEN plus aspirin group cirrhosis was less severe and carcinoma developed later than that in DEN group. Hepatic carcinoma occurred in four rats out of six in DEN group at the 16th week (P<0.05). In DEN plus aspirin group PCNA and cyclin D1 expressions were significantly lower than those in DEN group(P <0.05) , inhibiting effects on cyclin D1 expression was evidently great at 16th week (F=11.944,P<0.05) and inhibiting effect on PCNA was obvious from 12th to 16th week (F=5.696 and F=11.057, P<0.05 ). Conclusion Aspirin downregulates the expressions of PCNA and cyclin D1 in hepatic tissues and inhibits the genesis and development of DEN induced hepatic cancer of the rats.