Exogenous substance P induced autocrine of substance P in epidermal stem cells / 中华创伤杂志

ABSTRACT

Objective To study autocrine characteristics of substance P(SP)in epidermal stem cells(ESCs). Methods In vitro cultured ESCs was stimulated by exogenous SP.Then,the expressions of SP gene,protein and exogenous SP protein in ESCs and the supernatant fluid were detected by immunofluorescence,ELISA,Western blot and real-time PCR. Results After stimulation by exogenous SP,the expression of SP protein in ESCs and supernatant fluid was up-regulated and reached peak at 24th hour,with higher concentration than the added SP.The gene expression of SP was up-regulated to the peak 6 hours after stimulation by SP.Both expression of gene and protein remained unchanged after the natural killer cell receptor Was blocked even by stimulation with exogenous SP. Conclusion Anticrine of SP of ESCs can be induced by exogenous SP.