Proteomic analysis of lung protein expression in rats inhaled radon / 中华放射医学与防护杂志
Chinese Journal of Radiological Medicine and Protection
;
(12): 225-227, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-400323
ABSTRACT
Objective To detect the changes in protein profile of rats'lung inhaled radon using two- dimensional electrophoresis(2-DE)and MS.Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100,200 and 400 working level months(WLM).The total proteins of rats'lung were extracted and isolated by 2-DE.The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS.Resuits Comparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots,15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS.Conclusions The 2-DE image of radon groups'lung has changed,and the lung damage effect of radon is related to some proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Radiological Medicine and Protection
Year:
2008
Type:
Article
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