Prevalence of Ambler Class A Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 17-25, 2005.
Article
in Korean
| WPRIM
| ID: wpr-40114
ABSTRACT
BACKGROUND:
The aim of this study is to determine the nationwide prevalence of Ambler class A extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and to characterize genotypes of ESBLs.METHODS:
During the period of February through July, 2003, E.coli and K.pneumoniae isolates were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were tested by disk diffusion method, and ESBL-production was determined by the double-disk synergy test. MICs of beta-lactam antibiotics were tested by agar dilution method. Searches for bla TEM, bla SHV, bla CTX-M, bla PER-1, bla VEB, bla IBC, bla GES and bla TLA genes were performed by PCR amplification, and the genotypes of ESBLs were determined by direct nucleotide sequence analysis of amplified products.RESULTS:
Resistance rates of E.coli (n=246) and K.pneumoniae (n=239) isolates to ceftazidime were 8.5% and 20.1%, respectively. Most prevalent Ambler class A ESBL genotypes in E.coli isolates were bla CTX-M-15 (n=4) and bla CTX-M-3 (n=3), and each of bla CTX-M-14, bla SHV-12, and bla TEM-52 gene was also found in one isolate. Most prevalent ESBL genotypes in K.pneumoniae were bla SHV-12 (n=30) and bla CTX-M-3 (n=13), and bla CTX-M-14 (n=5). bla SHV-2a (n=3), bla SHV-5 (n=2), bla TEM-52 (n=1), bla GES-3 (n=2) genes were also found.CONCLUSION:
CTX-M-type ESBL-producing E.coli and K.pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Beta-Lactamases
/
Base Sequence
/
Ceftazidime
/
Polymerase Chain Reaction
/
Prevalence
/
Agar
/
Diffusion
/
Escherichia
/
Escherichia coli
/
Genotype
Type of study:
Prevalence study
/
Risk factors
Country/Region as subject:
Asia
Language:
Korean
Journal:
Korean Journal of Clinical Microbiology
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS