Detection of epstein-barr virus DNA by fluorescent quantitative PCR in children with infectious mononucleosis / 中国基层医药

ABSTRACT

Objective To investigate the application of EBV-DNA determineation by PCR and to analyze the clinical significance in 100 EBV positive patients.Methods EBV-DNA was detected in peripheral blood by PCR and fluorescence assay.The clinical features of 100 children with EBV-DNA positive were reviewed retrospectively.Results Patients had median ages of 3 years(range,1 month to 12 years).There were 65 patients under the age of 3,25 patients between 3 and 7,and the remained older than 7 years.Among these cases,the positive rate of EBV-DNA in IM group was 84.0%(84/100)and 5.0%(2/40)in the control group,the percent of positive in the former was significant higher than the later(P＜0.01).EBV-DNA concentration of patient with IM in early stage(75.52±175.11)×103copies/ml were higher than convalescence(0.67±2.27)×103copies/ml(P＜0.01).Conclusion The EBV-DNA detection by PCR is a quick,accurate and sensitive assay in diagnosis of EBV infection diseases.