Radioimmunoimaging of ~(131)Ⅰ-labelled anti-alveolar hydatid polyclonal antibodies in rats with Echinococcus multilocularis in vivo / 中国组织工程研究

ABSTRACT

BACKGROUND: Echinococcosis alveolaris were not easily discovered in prophasel Although used various kinds of imaging and immunity test, misdiagnosis rate of echinococcosis alveolaris was still high.OBJECTIVE: To study radioimmunoimaging of ~(131)Ⅰ-labelled anti-alveolar hydatid polyclonal antibody in the rat model with Echinococcus multilocularis in vivo.METHODS: Totally 36 Wistar rats were randomly divided into 3 groups. A group included 12 alveolar echinococcosis rats, B group included 12 normal rats, C group included 12 alveolar echinococcosis rats. Rats in the A and B group were injected intrapedtoneally 1 mL ~(131)Ⅰ-labelled anti-alveolar echinococcosis polyclonal antibodies (37 MBq), those in the C group were injected intraperitoneally I mL ~(131)Ⅰ-(37 MBq). All rats were imaged 24, 48, 72, 96 hours later with ECT to measure region of interest (ROI) of alveolar hydatid. The unit weight radioactive counts per minute of three group rats organs were measured after 96 hours, and the targetto-nontarget (TINT) ratios were calculated in A group rats.RESULTS AND CONCLUSION: In A group rat inoculated right lobe of liver alveolar echinococcosis, radioactive parts of the distribution could be seen. And ROI of alveolar echinococcosis were the highest at 72 hours. After dissection the locations were in line, and it was no significant radioactive liver distdbution in B and C group. Rats were sacrified after 96 hours, in vitro determination of radioactive counts show that A group echinococcus multilocularis was (18.610±3.401)x10~3 cpm/g, which was greater than organs except thyroid and gastric tissues (P ＜ 0.001). The rest of organize T/NT ratios were greater than 1.28 apart from the thyroid and gastric tissues. The difference of unit weight radioactive counts per minute of three group had no obviously significance except the polypide tissues (P ＞ 0.05). This proves that ~(131)Ⅰ-1abelled anti-alveolar echinococcosis polyclonal antibodY successfully combinated with alveolar echinococcosis.