Abnormity of SOD, MDA and COX-2 in colon of depression rats / 中华行为医学与脑科学杂志

ABSTRACT

Objective To investigate the abnormity of superoxide dismutase (SOD), malondialdehyde (MDA) and cyclooxygenase-2 (COX-2) in colon of rats caused by depression.Methods The chronic stress-induced model of depression of wistar rats was produced . The experimental animals were randomly divided into model control and normal control in the study. The levels of SOD and MDA were assayed with ultraviolet spectrophotometry. The expression of COX-2 in colonic tissue was detected with immunohistochemistry.Results Enhanced colon inflammatory response and oxidative stress were observed in the chronic stress-induced depression model of rats, which manifested as the significant increase of MDA, as well as the expressions of COX-2 in the colon tissue, while the colonic SOD activity was significantly decreased compared with the normal control (MDA 10.34±2.77 vs 2.55±0.70; COX-2 53.26±8.16 vs 4.87±1.05; SOD53.39±11.15 vs 84.45±22.31; P <0.01). Conclusion Depression can induce injuries on colon of rats, which was probably due to the abnormity of metabolism of oxygen free radical (OFR) and arachidonic acid.