Biological characteristics of human umbilical cord mesenchymal stem cells following cryopreservation / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1729-1733, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-402572
ABSTRACT
BACKGROUND:
An effective freezing-thawing technique is crucial for the clinical application of human umbilical cord mesenchymal stem cells(UC-MSCs).OBJECTIVE:
To investigate biological characteristics of UC-MSCs after cryopreservation.METHODS:
UC-MSCs were isolated from human umbilical cord and frozen in liquid nitrogen.The survival rate and the suppressive effect of γ-interferon(IFN-γ)of cryopreserved-thawed and fresh human UC-MSCs were compared.Furthermore,the multiple potentials and phenotype of UC-MSCs were estimated after cryopreservation.RESULTS ANDCONCLUSION:
There was no significant difference between cryopreserved-thawed and fresh human UC-MSCs on the survival rate and the suppressive effect of IFN-γ of peripheral blood mononuclear cells(PBMCs).After cryopreservation,human UC-MSCs had the potential differentiation and the phenotype of mesenchymal stem cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS