Immunohistochemical analysis of the expression of Hes1 in adult mouse brain / 中国组织工程研究

ABSTRACT

BACKGROUND:In recent years,it has been shown in studies that regulation of the expression of Has1 is very important to maintain neural stem cells and regulate its differentiation during the development of embryonic nervous system.Moreover,upregulation of Hes1 expression is required for quiescent adult flbroblasts resuming proliferation.This indicates that there is a close relationship between Hes1 and some potential stem cells in adult.Therefore,the study of Hes1 expression in neural stem cells and its relationship with adult neurogenasis is put on the agenda.However,the expression of Hes1 in adult brain is still not clear.OBJECTIVE:To observe the expression of Hea1 in adult mouse brain and the cell types of Has1 positive cells.METHODS:Totally 12 adult male C57BL/6 mice were randomly evenly divided into two groups by using the random number table.For the first group,the mouse brain was removed directly and immunohistochemical staining was used to detect the expression of Has1 in the adult mouse brain.For the second group,Brdu was injected intraperitoneally to every mouse with a dosage of 200 mg/kg,once a day,for 3 days.The mouse brain was removed at 4 days and then double staining was carded out to observe and analysis the cell types of Hes1 positive cells in hippocampus.RESULTS AND CONCLUSION:Hes1 was expressed in all the observed anatomical site where neural cells exist,Brdu positive cells nearly all expressed Hea1,NeuN positive cells all expressed Has1,whereas GFAP positive cells did not express Hes1 completely.Hes1 was expressed in all neurons and neural stem cells,and glial cells did not express Hes1.