Influence of astragalus injection on in vitro cultured olfactory ensheathing cell proliferation and neurotrophic factor secretion / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 2564-2567, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-402603
ABSTRACT
BACKGROUND:
Astragalus or olfactory ensheathing cell(OECs)transplantation alone can promote survival of various neurons and functional improvement of injured spinal cord.The clinical effect of astragalus in combination with OECs transplantation remains unclear.OBJECTIVE:
To observe the effect of astragalus on the proliferation of OECs and the secretion of neurotrophic factors.METHODS:
OECs of SD rats,24 hours old,were isolated and cultured,and identified by immunohistochemistry.OECs at culture day 8 were seeded in polylysine-coated 96-well plate and incubated in 5% CO2 at 37℃ for 24 hours,followed by 2 mg/L,20 mg/L,200 mg/L,2 g/L,20 g/L astragalus injection for 24 hours.MTT and flow cytometry were used to assess the effects of astragalus in promoting OECs proliferation;enzyme linked immunoassay(ELISA)was used to measure the content of neurotrophic factors.Serum culture medium was served as negative control.RESULTS ANDMETHODS:
Compared with the negative control group,2 and 20 mg/L astragalus injection significantly promoted OECs proliferation(P<0.05,P<0.01).The result of flow cytometry showed that astragalus could promote OECs in G1 stage and reduce the percentage of cells in S and G2 stages,but the difference was not significant(P>0.05).However,astragatus injection at each mass concentration significantly reduced the number of OECs(P<0.05).Moreover,2 mg/L astragalus injection significantly promoted in vitro neurotrephic factor levels in OECs.Results show that astragalus injection in combination with OECs can promote recovery of spinal cord injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS