Detection and separation of side population cells in haematological tumor cell lines / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1821-1824, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-402697
ABSTRACT
BACKGROUND:
Side population(SP)cells,with varied contents,are widely distributed in adult tissues,embryos,and certain tumor cells.Haematological tumor is one of the main pathological conditions,which endangers human life.Thus,it is important to recognize SP cells in haematological tumor cell lines.OBJECTIVE:
To identify whether hematologic tumor cell lines contain SP cells,and to explore a stable detection and separation methods.METHODS:
Cells with the characteristics of stem cells being capable of fluorescent dye Hoechst33342 were isolated by flow cytometry;whether there were SP cells in logarithmic growth period of NB4,Raji,K562/ADM and K562 or not were detected.After sorting K562 subpopulations,the purity of sorted cells was detected.RESULTS ANDCONCLUSION:
After Hoechst33342 staining,flow cytometry results showed that the SP cells appeared in the haematological tumor NB4,Raji,K562/ADM and K562 cell lines,which accounted for 0.8%,2.7%,1.3% and 2.7%,respectively.These cells could be blocked by Verapamil.The purity was greater after a second detection of SP and Non SP cells in K562 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS