The effects of Metacycloprodigiosin on tumor metastasis-associated cell behavior in vitro / 中国药理学通报

ABSTRACT

Aim To investigate the effects of Metacycloprodigiosin(MP) on tumor metastasis-associated cell behavior in vitro.Methods The effects of MP on the proliferation of HUVEC and 4TO7 cells were observed by MTT assay.Migration abilities of HUVEC and 4TO7 cells were measured by wound healing assay.Tube formation of HUVEC in the presence of MP for 10 hours was evaluated on matrigel basement membrane matrix.The abilities of adhesion and invasion of 4TO7 with MP for 24 hours were measured quantitatively by cell adhesion and transwell assay.Results MP did not affect proliferation of HUVEC and 4TO7 cells in concentration less than 10 μmol·L-1.MP inhibited HUVEC cells migration and formation of the tube in the concentrations of 0.01 to 1 μmol·L-1.In these concentrations MP could also inhibit 4TO7 cells migration,adhesion and invasion.Conclusion sThese results demonstrate that MP effectively inhibits tumor metastasis in vitro.All these findings suggest its potential as an anti-tumor metastasis compound in vivo.