Isolation and cultivation of goat temporomandibular joint disc cells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 165-168, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-403365
ABSTRACT
Objective:
To study the isolation, culture and identification of the TMJ cells and to observe the biological characteristics of cultured fibrochondrocytes.Methods:
The TMJ discs were dissected from two 1 month goats under sterile conditions and were digested with collagenase. The cells were collected. Morphological changes and attachment efficiency were constantly observed under phase-contrast microscope. Immunohistochemical staining for type I collagen as well as toluidine blue staining were performed. Ultrastructures of the TMJ cells were observed under transmission electron microscope.Results:
Most of the primary fibrochondrocytes presented a short spindle-shape while the rest showed polygon-shape. On the 7th day, the perliferating fibrochondrocytes started to contact each other to form a monolayer covering the bottom of the incubation disc. Immunohistochemical staining of type I and toluidine blue staining exhibited positive results. The fibrochondrocytes cytoplasms were rich in mictochondria and endoplasm reticulum.Conclusion:
The fibrochondrcytes isolated from one-month-old goat TMJ disc have good proliferation ability in vitro and cells from passage 1 to 3 might be used as seed cells for TMJ disc tissue engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2010
Type:
Article
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