Preparation and Application of Fluorescein Isothiocynate Fluorophore Nano-composites / 分析化学
Chinese Journal of Analytical Chemistry
;
(12): 202-206, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-403817
ABSTRACT
Nano-composite fluorescence with silica shell had been prepared by improving St(o)ber route. The precursor was obtained via chemical reaction of fluorescein isothiocyanate(FITC) and 3-aminopropyltrietho-)xysilane(APTEOS)). Then, the controlled hydrolysis and condensation of tetraethylorthosilicate(TEOS) leaded) to the formation of organic-inorganic nano-composites. The transmission electron microscope(TEM) showed that the nano-composites with a diameter of about 70 nm were uniform, spherical and mono-dipersed. The nano-composites fluorescence significantly reduced dye leaching and had strong fluorescence when they were by water for several times. Labeled bovine scrum albumin(BSA) with the nano-composites fluorescence showed green fluorescence measured by laser scanning confocal microscope.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Analytical Chemistry
Year:
2010
Type:
Article
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