Effect of probiotics on intestinal flora disturbance and bacterial translocation in mice with spontaneous colitis / 上海交通大学学报（医学版）

ABSTRACT

Objective To investigate the effect of Lactobacillus plantarum(LP)on intestinal flora and bacterial translocation in mice with spontaneous inflammatory bowel disease(IBD). Methods Interleukin 10 knockout mice(IL-10~(-/-))were used as models of IBD.Eight-week old female mice were randomized to control group, IL-10~(-/-)group and IL-10~(-/-)+LP group.IL-10~(-/-)+LP group received 0.5 mL LP(1.0×10~9CFU/mL)per day for 4 weeks,and the other groups received 0.5 mL Ringer buffer.Intestinal flora including Bifidobacteria,Lactobacilli,Enterobacteriaceae and Clostridium perfringens in the feces and bacterial translocation in mesenteric lymph nodes and spleens were detected. Results The contents of Bifidobacteria and Lactobacilli significantly decreased in the intestine of IL-10~(-/-)mice,while those of Enterobacteriaceae and Clostridium perfringens significantly increased,and the bacterial translocation significantly increased.Four weeks after LP treatment, the disturbed intestinal flora was restored, and the bacterial translocation decreased. Conclusion LP administration can modulate the imbalance of intestinal flora and decrease the bacterial translocation,thus enhance intestinal barrier function in mice with IBD.