C/EBPα is involved in transcriptional regulation of mVGLUT2 gene expression / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 142-145, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-404168
ABSTRACT
AIM:
The main purpose of this study is to investigate the regulatory role of C/EBPα in mouse vesicular glutamate transporter 2(mVGLUT2) gene expression.METHODS:
The promoter region of mVGLUT2 was cloned to PGL3-basic vector. Site-direction mutation was used to identify the CCAAT-enhancer-binding protein α(C/EBPα) binding site. The promoter activity was observed by luciferase system. The binding between C/EBPα protein and mVGLTU2 promoter region was determined by EMSA. The C/EBPα gene expression was inhibited by its specific siRNA.RESULTS:
mVGLUT2 promoter activity decreased about 50% after mutation of C/EBPα binding site. EMSA showed that C/EBPα protein bound onto mVGLUT2 promoter region. Meanwhile, when C/EBPα gene expression was inhibited by its specific siRNA, mVGLUT2 promoter activity, mRNA level and protein level were decreased about 60%, 40% and 45%, respectively.CONCLUSION:
C/EBPα is involved in the regulation of mVGLUT2 gene expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2010
Type:
Article
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