Effect of Advanced Glycation End Products on Oxidative Stress and Senescence of Trabecular Meshwork Cells
Korean Journal of Ophthalmology
;
: 123-131, 2012.
Article
in English
| WPRIM
| ID: wpr-40419
ABSTRACT
PURPOSE:
To investigate the effect of advanced glycation end products (AGE) on oxidative stress and cellular senescence in cultured human trabecular meshwork cells (HTMC).METHODS:
Primarily cultured HTMC were exposed to 0, 10, 50, 100, 200 microg/mL of glycated bovine serum albumin (G-BSA) for 5 days. Also co-exposed were L-arginine, sepiapterin, and antioxidant N-acetylcysteine (NAC). Cellular survival and production of nitric oxide (NO), superoxide, and reactive oxygen species were assessed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay, Griess assay, cytochrome c assay, and dichlorofluorescin diacetate assay, respectively. Senescence-associated beta-galactosidase staining was performed to quantify the degree of cellular senescence.RESULTS:
G-BSA decreased cellular survival, NO production, and increased superoxide production significantly in a dose-dependent manner. The effects of G-BSA were abolished with co-exposure of L-arginine, sepiapterin, and NAC. G-BSA enhanced cellular senescence accompanied by increased production of reactive oxygen species. G-BSA-induced cellular senescence was suppressed by application of L-arginine, sepiapterin, and NAC.CONCLUSIONS:
AGE enhances cellular senescence of HTMC accompanied with increased oxidative stress. AGE-induced oxidative stress and cellular senescence could be delayed by application of anti-oxidants.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arginine
/
Acetylcysteine
/
Pterins
/
Trabecular Meshwork
/
Serum Albumin, Bovine
/
Cell Survival
/
Cells, Cultured
/
Cellular Senescence
/
Reactive Oxygen Species
/
Apoptosis
Limits:
Humans
Language:
English
Journal:
Korean Journal of Ophthalmology
Year:
2012
Type:
Article
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