Effects of gastrin on NF-κB signaling pathway in human colon cancer cells / 中国药理学通报

ABSTRACT

Aim To investigate the effects of gastrin-17 on NF-κB signaling pathway in human colon cancer cells-Colo320WT.Methods All subjects were divided into four groups control, gastrin-17,gastrin-17+L365,260 (gastrin-17 receptor blocker) and L365,260 group.Concerning gastrin-17 group,Colo320WT cells were treated by gastrin-17 for 12 h.Concerning gastrin-17+L360,265 group, Colo320WT cells were pretreated by L365,260 for 30 min and then were treated by gastrin-17 for 12 h.Concerning L365,260 group,Colo320WT cells were pretreated by L365,260 for 30 min.NF-κB DNA binding activity was determined by electrophoretic mobility shift assay (EMSA). Expressions of uPA protein and mRNA were assayed by Western blot and RT-PCR, respectively.Results NF-κB DNA binding activity increased more greatly in the Colo320WT cells stimulated by gastrin than that of the control group. And the expressions of uPA protein and mRNA were up-regulated more markedly than those of the control group. The above responses resulting from gastrin-17 were partly blocked by gastrin-17 receptor blocker, L365,260.Conclusion Gastrin-17 may activate NF-κB signaling pathway in human colonic cancer cells Colo320WT.