Inhibitory effect of cochinchina momordica seed ethanol extract on proliferation of melanoma B16 cells and its possible meachanism / 中国肿瘤生物治疗杂志

ABSTRACT

Objective: To investigate the effect of cochinchina momordica seed ethanol extract (CMSEE) on the proliferation of melanoma B16 cells and the underlying mechanism. Methods: MTT and clone formation assay were used to assess the effect of CMSEE on the growth of B16 cells. Morphological changes of B16 cells were observed under phase-contrast microscope and Giemsa staining. Cell cycle and apoptosis rate were examined by flow cytometry (FCM). The effect of CMSEE on melanin production and tyrosinase activity of B16 cells was assessed by colorimetry. The effect of CMSEE on the expression of C-myc, P38, and Tyr genes was examined by RT-PCR. Results: CMSEE (10-100 mg/L) inhibited the proliferation of B16 cell in a dose-and time-dependent manner. After treatment with 10-40 mg/L CMSEE, B16 cells showed typical differentiation morphology, and melanin production and tyrosinase activity were increased. B16 cells treated with 100 mg/L CMSEE showed apoptotic morphology, decreased melanin production and tyrosinase activity. B16 cell number in G_0/G_1 phase was significantly increased (P<0.01); C-myc mRNA expression was down-regulated, and P38, Try mRNA expression was up-regulated in B16 cells after treatment with 10-40 mg/L CMSEE. Conclusion: CMSEE can markedly inhibit the proliferation of melanoma B16 cells, which is related to induction of differentiation and promotion of apoptosis of B16 cells.