Role of EP_2 in synaptic transmission in dentate gyrus of hippocampus / 西安交通大学学报(医学版)

ABSTRACT

Objective To evaluate the role of EP_2 in synaptic transmission in dentate gyrus of hippocampus. Methods Field excitatory post-synaptic potentials (fEPSP) were recorded at the perforated path-granule neurons in dentate gyrus in vitro. Results ① Butaprost, an agonist of EP_2, enhanced the synaptic transmission in dentate gyrus and decreased the paired-pulse ratio, and these effects were reversed by bath application of AH6809 (EP_2 antagonist). ② Application of Forskolin alone or with AH6809 elevated the slope of fEPSP. ③ The Butaprost-induced responses were mediated via PKA, ERK and IP_3 signal pathways. Conclusion Multiple signal pathways were involved in the EP_2 activation-mediated enhancement of synaptic transmission.