Biocompatibility evaluation of polyvinyl alcohol-gelatin esterified hydrogel for wound dressing

Yulan OU

ABSTRACT

BACKGROUND:

Functional hydrogel Is a new biomaterial, which considered as wound dressing should be generally evaluated according to biological standard to prove the great biocompatibility prior to clinical application.

OBJECTIVE:

To evaluate the biocompatibility of a new kind of wound dressing, polyvinyl alcohol-gelatin esterified (PGE) hydrogel.DESIGN, TIME AND

SETTING:

A materials study was performed at Central Laboratory, College of Public Health, University of South China from April 2008 to June 2009.MATERIALS Fibroblast strain of NCTC L-929 mice was provided by Medical College of Sun Yat-sen University; experimental animals were provided by Animal Faulty of Southern China University; polyvinyl alcohol and gelatin were provided by Chemical Agent Engineering Developing Center, Guangdong.

METHODS:

A 10-g polyvinyl alcohol was generally dissolved in 100 mL water and the mixture was added with 5 g gelatin at 70 °C. Additionally, hydrochloric acid was added and fully stirred for esterification reaction. The dispersion solution was inserted into the tooting and transformed into membrane-like products which were sectioned into 1-mm round and thin sections. Toxicity test, hemolytic test, and whole-body acute-term toxicity test, and sensitization test of PGE were performed according to national GB/T16886 standard.MAIN OUTCOME

MEASURES:

Biocompatibility of PGE hydrogel.

RESULTS:

Toxicity reaction of PGE hydrogel was grade 0-1, and hemolytic ratio was 5%. Following an injection of PGE hydrogel, mice did not have death, convulsion, paralysis, respiratory depression, diarrhea, and decreased body mass. Seventy-two hours later, erythema and edema were not observed on both sides of spine of mice.

CONCLUSION:

The PGE hydrogel has a good biocompatibility and can be used for wound dressing on clinic.