MucA mutation and its alginate-production in clinically isolated Pseudomonas aeruginosa / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1196-1201, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-404794
ABSTRACT
Objective To determine the characterization of mucA gene mutation in clinically isolated Pseudomonas aeruginosa (P.aeruginosa), and the relation between mucA mutation and the mucoid phenotype.Methods A total of 58 strains of P.aeruginosa were collected. Of them,8 were nonmucoid phenotype and 50 were mucoid phenotype.We detected mucA mutations with PCR-SSCP and sequencing analysis. Alginate was examined by colorimetry. Results All strrains had mucA mutations (100%), 16 of the 50 (32%) isolates contained mucA mutations that could alter the encoding sequence of amino acids, and the rate in nonmucoid isolates was 0. Fourteen mutation sites were found, 5 of which could alter the encoding sequence of amino acids, and the others were silent mutations. The alginate concentration of mucoid P.aeruginosa was higher than the nonmucoid P.aeruginos(P<0.01). The alginate concentration of the isolates which contained mucA mutations that could alter the encoding sequence of amino acid was higher than the strains only with silent mutations (P<0.01).Conclusion mucA mutation correlates with the alginate production and phenotype of bacterial colonies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2009
Type:
Article
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