Effect of basic fibroblast growth factor on the proliferation and differentiation of human preadipocytes / 中国组织工程研究

ABSTRACT

BACKGROUND:Previous studies have shown that basic fibroblast growth factor (bFGF) can enhance fat survival rate by promoting vessel regeneration of transplanted fat.However,there is no clear conclusion addressing its effects on adipocytes.OBJECTIVE:To investigate the effect of bFGF on the proliferation and differentiation of human preadipocytes.DESIGN,TIME AND SETTING:Cytological in vitro comparison observation was conducted at the Medical College of Chinese People's Armed Police Force from March 2006 to December 2007.MATERIALSTotally 10 samples of adipose tissue from patients undergoing abdominal liposuction were used in this study,including 1 male and 9 females,aged 21-45 years.METHODS:The human preadipocytes were obtained from human abdominal fat particles by isolation,culture and natural purity.The experiment was divided into bFGF and control groups.Cells in the control group were incubated in DMEM.Cells in the bFGF group were incubated in DMEM supplemented with 100 ng/L bFGF.MAIN OUTCOME MEASURES:Morphology of cultured cells was studied to determine the growth curve at 0,3,6,9,12,15 days following culture by inverted phase contrast microscope.Fat aggregation was observed in cells using oil red O staining.Absorbance value was examined using enzyme-linked immunosorbent assay.RESULTS:The preadipocytes represented the spindle-like character with strong proliferation.During 1 to 15 days,the preadipocytes proliferated into monolayers.Some cells became single bubble cells with lipid droplets deposited,close to maturity.The cells in bFGF group had no significant differences with the control group in cell morphology,but the amount of cells in bFGF group was 44% more than the control group.Following oil red O staining,lipid droplet was found in the preadipocytes,showing dark red.Following 15 days of incubation,the concentration of lipid droplet reached a peak.The absorbanca of bFGF group was increased 300% compared with the control group (P ＜ 0.05).CONCLUSION:The bFGF not only promote the proliferation of human preadipocytes,but also induce the differentiation of preadipocytes into mature adipocytes.