Expression of transforming growth factor-β and metalloproteinases in joint capsule of frozen shoulder / 上海交通大学学报（医学版）

ABSTRACT

Objective To explore the role of transforming growth factor (TFG) -β and matrix metalloproteinases( MMPs) in the development of frozen shoulder. Methods Twenty-four patients who underwent shoulder arthroscopy were included, and were divided into frozen shoulder group (n = 12) and control group ( n = 12; n = 2 for shoulder instability, n = 5 for rotator cuff tear and n = 5 for subacromial impingement) . Joint capsule tissues at the rotator cuff interval were obtained, and the expression of TGF-β, MMP-1, MMP-2, MMP-3, MMP-9 and MMP-12 mRNA and protein was detected by Real-time PCR and Western blotting, respectively. Results The expression of TGF-p mRNA in frozen shoulder group and control group was 3.36 × 10~4 ±2.18 × 10~3 and 1.85 × 10~4 ±3.31×10~3, respectively, the expression of TGF-p protein was 1.55 ± 0.33 and 1.13 ±0.21, respectively, and there were significant differences between these two groups (P < 0.05). The expression of MMP1, MMP2, MMP3, MMP9 and MMP12 mRNA and protein in frozen shoulder group was significantly higher than that in control group(P <0.05) . Conclusion The higher expression of TGF-β and MMPs in joint capsule of frozen shoulder may be associated with the development of frozen shoulder.