Two-dimentional liquid phase chromatographic proteome profiles in Uighur women with uterine cervix cancer / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 624-629, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-406294
ABSTRACT
Objective To establish differential protein expression profile specific for serum proteome of cervical carcinoma in Uighur women by protein 2-dimensional liquid phase chromatogra-phy. Methods We collected and prepared sera of cervical carcinoma from Uighur women, and sepa-rated and processed data using 2-dimensional liquid phase chromatography system specific for protein (ProteomeLabTM PF-2D ). Results A differential expression profile of serum proteome based on characteristics of protein isoelectrie point and hydrophobic features was successfully established. Fifty-six differentially expressed protein spots were found, among which there was an obvious difference in 12 peaks. Conclusion Two-dimensional liquid phase chromatography is an effective and feasible method to establish differential expression profile of serum proteome and to offer a new way for the screening of serum protein markers of Uighur women with cervical carcinoma.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2009
Type:
Article
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