Effect of adriamycin nanoparticles conjugated with hyaluronic acid on oral squamous cell carcinomas in vitro / 中国组织工程研究

ABSTRACT

ACKGROUND Adriamycin (ADM) can specifically conjugate with receptor. In particular, ADM nanopartides play a target role in decreasing the cytotoxicity.OBJECTIVE: To investigate the targeting effect of addamycin nanoparticles conjugated with hyaluronic acid (ADM-HA-SSL) on oral squamous carcinomas calls in vitro.DESIGN, TIME AND SETTING: An in vitro contrast observation was performed in College of Madne Life Science, Ocean University of China from January to July 2008. MATERIALS Oral squamous carcinomas calls were sincerely presented by Professor Chen from the Ninth People's Hospital of Shanghai; ADM-HA-SSL (drug loading 156 mg/L) was sincerely presented by Professor Liu from College of Marine Life Science, Ocean University of Chine.METHODS: Oral squamous carcinomas cells were cultured with 0.5, 1.0, 5.0, and 10.0 mg/L ADM-HA-SSL. MTT assay was used to detect the targeted cytotoxicity of ADM-HA-SSL against oral squamous cell carcinomas. With the concentrations of 5.0 and 10.0 mg/L, call apoptosis was ascertained by call flow cytometry after 6, 12, 24 and 48 hours. MAIN OUTCOME MEASURES: Cell survival rate and apoptosis rate.RESULTS: At 24 and 48 hours after induction, cytotoxicity assay revealed that the effect of ADM-HA-SSL was superior to that of free ADM (t=5.78-42.05, P < 0.01). The results of flow cytometry showed that the apoptosis rate was enhanced with the increase of the time (F=4 200.40, 4 775.36, P < 0.01), and the rate was also increased at the same time point with the increase ofconcentration (t=12.06-20.08, P < 0.05).CONCLUSION: ADM-HA-SSL can specifically recognize oral squamous carcinomas cells and deliver adriamycin into the cells. And the effect is enhanced by the time prolonging.