Associatian of angiotensin converting enzyme gene polymorphism and its serum levels with cerebral infarction / 国际检验医学杂志

ABSTRACT

ObjectiveTo investigate the association of angiotensin converting enzyme(ACE) gene polymorphism and its serum levels with cerebral infarction, and further to explore its possible mechanism. MethodsEighty-four patients with cerebral infarction(cerebral infarction group) and 74 healthy controls(healthy control group) underwent the determination of ACE gene insertion/deletion polymorphism by polymerase chain reaction(PCR) and agarose gel electrophoresis(AGE). The serum levels of ACE were measured simultaneously. The difference in distribution of ACE gene polymorphism was compared between cerebral infarction group and ealthy control group. ResultsThe frequency of DD genotype in cerebral infarction group was significantly higher than that of healthy control group(50% vs 28% ,P<0.01); so was the frequency of D allele(64% vs 46% ,P<0.05). ACE gene polymorphism was associated with the serum level of ACE. A significant difference in serum ACE level was observed among three ACE genotypes. The highest serum ACE level or activity was in deletion homozygote(genotype DD), followed by heterzygote(genotype ID) and insertion homozygote(genotype Ⅱ)(P<0.01). ConclusionThe polyrnorphism of ACE gene and serum level of ACE are associated with cerebral infarction. The DD gene type of ACE and D allele may be the risk factor for cerebral infarction.