Protection of nitric oxide for hepatic steatosis caused by total parenteral nutrition in rats / 中华普通外科杂志

ABSTRACT

Objectives　To investigate the effect of nitric oxide(NO) on hepatic steatosis caused by total parenteral nutrition(TPN) in rats.Methods　Thirty Wistar rats were randomly divided into five groups Group A,normal control; Group B,TPN group;Group C,TPN plus L-arginine; Group D,TPN plus NG-nitrio-L-arginine methyl ester(L-NAME，NOS inhibitor); Group E,TPN plus L-arginine and L-NAME.At the seventh day,liver function tests were performed and the livers were resected to test the lipid and nitric oxide content,and nitric oxide synthase activity.Results　The hepatic lipid content 〔triglycerid(μmol/g),cholesterol(μmol/g)〕in group B significantly increased compared with group A （39.3±2.4、13.1±1.1 vs.6.9±0.8、5.6±0.6）(P＜0.05).That was higher in group D（49.7±6.2、14.1±1.7）than in group B(P＜0.05),whereas that was lower in group C（18.2±4.4、7.3±3.1）than in group B(P＜0.05).The activity and distribution of NOS in the liver were associated with the content and distribution of hepatic lipid.Conclusions　NO produced by the liver may protect hepatic steatosis caused by total parenteral nutrition in rats.