A sensitive HPLC method for determination of amphotericin B and itraconazole in cerebrospinal fluid and its application to patients with cryptococcal meningitis / 复旦学报(医学版)
Fudan University Journal of Medical Sciences
;
(6): 866-872, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-406886
ABSTRACT
Objective To establish a sensitive, rapid and simple high-performance liquid chromatography (HPLC) method for the determination of amphotericin B (AraB) and itraconazole (ITZ) in human cerebrospinal fluid (CSF). Methods Solid-phase extraction (SPE) was involved in sample preparation in this method, followed by reverse-phase separation under two different chromatographic systems. Results The tinearity for both AraB and ITZ ranged from 5 ng/mL to 100 ng/mL and the inter-day and intra-day RSD were both below 7.6% at the concentrations of 20,50 and 100 ng/mL. Conclusions The method we established has been applied to the therapeutic drug monitoring on cryptococcal-meningitis-infected patients who were given comedication of these two drugs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Fudan University Journal of Medical Sciences
Year:
2008
Type:
Article
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