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Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons / 神经解剖学杂志
Article in Zh | WPRIM | ID: wpr-407485
Responsible library: WPRO
ABSTRACT
To investigate the effects of didannsine(ddI)on the morphological alterations of dorsal root ganglion(DRG)neurons,dissoci-ated DRG cells from rat embryo were studied.DRG cells were cultured for 3 days and then treated with ddI for additional 3 claysin differ-ent concentrations(1μg/ml,5 μg/ml,10μg/ml and 20 μg/ml,respectively).Afarthat,DRG cells were processedformicrotubule as-soeiated protein 2(MAP2)labeling and observed under confocal laser scanning microscopy(CLSM).The results showed that both thenumber and length of neurites of the DRG cells after exposed to ddl significantly down-regulated in a dose-dependentmanner compared withcontrol group,thus suggesting that ddI may have inhibitory effects on neufite regeneration and outgrowth in dissociated DRG cultures.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Neuroanatomy Year: 2008 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Neuroanatomy Year: 2008 Type: Article